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Part 135 Drug and Alcohol Program Requirements: A Practical Compliance Guide

Set up and maintain a DOT-compliant testing program with clear evidence trails and recurring controls.

Navlyt Editorial TeamNavlyt Editorial TeamNavlytPublished 3/20/2026Updated 3/20/20269 min read
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Part 135 Drug and Alcohol Program Requirements: A Practical Compliance Guide

Program foundation: policy, enrollment, governance

A compliant program starts with documented policy, safety-sensitive employee enrollment, and role clarity across HR, operations, and leadership.

Use our charter startup compliance setup guide to sequence initial controls and avoid missed onboarding steps.

Random testing controls and evidence

Your program should retain test selection logic, event records, result handling, and corrective action history in one evidence trail.

Centralize evidence and reminders in Navlyt Compliance so random testing deadlines do not depend on manual calendar checks.

Tip

Run a quarterly internal audit of random pool coverage and missing signatures.

Supervisor reasonable suspicion training

Supervisors need recurring training and acknowledgment records. In many findings, training occurred but proof was incomplete or hard to retrieve.

Tie training completions to role-based onboarding and annual refresher milestones.

Compliance warning

If documentation is incomplete, inspectors may treat the control as not performed.

Implementation path for small teams

Start with one standardized evidence pack template and reuse it for every event type. Simplicity improves consistency and reduces review time.

To operationalize quickly, launch your workflow from the Navlyt trial and pricing page and assign control owners in week one.

Navlyt tracks this automatically

Turn recurring compliance work into automated tasks and evidence trails.

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Key takeaways

  • Document governance and enrollment from day one.
  • Maintain full evidence trails for random testing events.
  • Train supervisors on schedule and store acknowledgment proofs.
  • Use standardized templates to reduce documentation drift.

Author

Navlyt Editorial Team

Navlyt Editorial Team

Navlyt

Written by the Navlyt team. Guides reference the specific regulations they discuss and are general information, not legal or regulatory advice — confirm requirements for your operation with your FSDO or compliance officer.

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